Publications
Selected Recent Publications
* Indicates trainee in the lab: medical student, graduate student, resident, clinical fellow, postdoctoral fellow, research associate, or research assistant.
1. *Sahajpal NS, Mondal AK, Ananth S, Pundkar C, Jones K, Williams C, Fee T, Weissman A, Tripodi G, Oza E, Gavrilova-Jordan L, Omar N, Hastie AR, DuPont BR, Layman L, Chaubey A, Kolhe R. Optical Genome Mapping and Single Nucleotide Polymorphism Microarray: An Integrated Approach for Investigating Products of Conception. Genes (Basel). 2022 Apr 3;13(4):643. doi: 10.3390/genes13040643. PMID: 35456449; PMCID: PMC9026980.
2. *Forgacs D, Silva-Moraes V, Sautto GA, Hanley HB, Gattiker JL, Jefferson AM, Kolhe R, Ross TM. The Effect of Waning on Antibody Levels and Memory B Cell Recall following SARS-CoV-2 Infection or Vaccination. Vaccines (Basel). 2022 Apr 29;10(5):696. doi: 10.3390/vaccines10050696. PMID: 35632452; PMCID: PMC9143792.
3. Simon V, Kota V, Bloomquist RF, Hanley HB, Forgacs D, Valdez R, Cobey S, Reed EF, Kolhe R, Thomas PG, Schultz-Cherry S, Ross TM, Krammer F. PARIS and SPARTA: Finding the Achilles' Heel of SARS-CoV-2. mSphere. 2022 Jun 29;7(3):e0017922. doi: 10.1128/msphere.00179-22. Epub 2022 May 19. PMID: 35586986; PMCID: PMC9241545.
4. *Ahluwalia P, Mondal AK, Ahluwalia M, Sahajpal NS, Jones K, Jilani Y, Gahlay GK, Barrett A, Kota V, Rojiani AM, Kolhe R. Clinical and molecular assessment of an onco-immune signature with prognostic significance in patients with colorectal cancer. Cancer Med. 2022 Mar;11(6):1573-1586. doi: 10.1002/cam4.4568. Epub 2022 Feb 9. PMID: 35137551; PMCID: PMC8921909.
5. Cantu, N., Vyavahare, S., Kumar, S., Chen, J., Kolhe, R., Isales, C. M., Hamrick, M., & Fulzele, S. (2022). Synergistic Effects of Multiple Factors Involved in COVID-19-dependent Muscle Loss. Aging and disease, 13(2), 344–352. https://doi.org/10.14336/AD.2021.0817
6. *Sahajpal, N. S., Mondal, A. K., Ananth, S., Njau, A., Jones, K., Ahluwalia, P., ... & Kolhe, R. (2022). Clinical validation of a multiplex PCR-based detection assay using saliva or nasopharyngeal samples for SARS-Cov-2, influenza A and B. Scientific reports, 12(1), 1-7. https://doi.org/10.1038/s41598-022-07152-0 PMID: 35241679
7. *Sahajpal NS, Jill Lai CY, Hastie A, Mondal AK, Dehkordi SR, van der Made CI, Fedrigo O, Al-Ajli F, Jalnapurkar S, Byrska-Bishop M, Kanagal-Shamanna R, Levy B, Schieck M, Illig T, Bacanu SA, Chou JS, Randolph AG, Rojiani AM, Zody MC, Brownstein CA, Beggs AH, Bafna V, Jarvis ED, Hoischen A, Chaubey A, Kolhe R; COVID19hostgenomesv Consortium. Optical genome mapping identifies rare structural variations as predisposition factors associated with severe COVID-19. iScience. 2022 Feb 18;25(2):103760. doi: 10.1016/j.isci.2022.103760. Epub 2022 Jan 10. PMID: 35036860; PMCID: PMC8744399.
8. Kolhe R, Ghilzai U, Mondal AK, Pundkar C, Ahluwalia P, Sahajpal NS, Chen J, Isales CM, Fulcher M, Fulzele S. Nanostring-Based Identification of the Gene Expression Profile in Trigger Finger Samples. Healthcare (Basel). 2021 Nov 20;9(11):1592. doi: 10.3390/healthcare9111592. PMID: 34828637; PMCID: PMC8619339.
9. *Ahluwalia P, Mondal AK, Sahajpal NS, Rojiani MV, Kolhe R. Gene signatures with therapeutic value: emerging perspective for personalized immunotherapy in renal cancer. Immunotherapy. 2021 Nov 10. doi: 10.2217/imt-2021-0187. Epub ahead of print. PMID: 34753298.
10. *Sahajpal NS, Mondal AK, Njau A, Petty Z, Chen J, Ananth S, Ahluwalia P, Williams C, Ross TM, Chaubey A, DeSantis G, Schroth GP, Bahl J, Kolhe R. High-Throughput Next-Generation Sequencing Respiratory Viral Panel: A Diagnostic and Epidemiologic Tool for SARS-CoV-2 and Other Viruses. Viruses. 2021 Oct 14;13(10):2063. doi: 10.3390/v13102063. PMID: 34696495; PMCID: PMC8540770.
11. Kolhe R, Sahajpal NS, Vyavahare S, Dhanani AS, Adusumilli S, Ananth S, Mondal AK, Patterson GT, Kumar S, Rojiani AM, Isales CM, Fulzele S. Alteration in Nasopharyngeal Microbiota Profile in Aged Patients with COVID-19. Diagnostics (Basel). 2021 Sep 5;11(9):1622. doi: 10.3390/diagnostics11091622. PMID: 34573964; PMCID: PMC8467337.
12. *Ahluwalia P, Ahluwalia M, Mondal AK, Sahajpal NS, Kota V, Rojiani MV, Kolhe R. Natural Killer Cells and Dendritic Cells: Expanding Clinical Relevance in the Non-Small Cell Lung Cancer (NSCLC) Tumor Microenvironment. Cancers. 2021 Aug 11;13(16):4037. doi: 10.3390/cancers13164037. PMID: 34439191; PMCID: PMC8394984.
13. *John G, Sahajpal NS, Mondal AK, Ananth S, Williams C, Chaubey A, Rojiani AM, Kolhe R. Next-Generation Sequencing (NGS) in COVID-19: A Tool for SARS-CoV-2 Diagnosis, Monitoring New Strains and Phylodynamic Modeling in Molecular Epidemiology. Curr Issues Mol Biol. 2021 Jul 30;43(2):845-867. doi: 10.3390/cimb43020061. PMID: 34449545.
14. Moore JX, Gilbert KL, Lively KL, Laurent C, Chawla R, Li C, Johnson R, Petcu R, Mehra M, Spooner A, Kolhe R, Ledford CJW. Correlates of COVID-19 Vaccine Hesitancy among a Community Sample of African Americans Living in the Southern United States. Vaccines (Basel). 2021 Aug 8;9(8):879. doi: 10.3390/vaccines9080879. PMID: 34452004; PMCID: PMC8402307.
15. Jones L, Bakre A, Naikare H, Kolhe R, Sanchez S, Mosley YC, Tripp RA. Isothermal amplification and fluorescent detection of SARS-CoV-2 and SARS-CoV-2 variant virus in nasopharyngeal swabs. PLoS One. 2021 Sep 17;16(9):e0257563. doi: 10.1371/journal.pone.0257563. PMID: 34534259; PMCID: PMC8448339.
16. *Sahahjpal NS, Mondal AK, Ananth S, Jones K, Chaubey A, Kolhe R. COVID-19 diagnostic assay sensitivity: lessons for the upcoming wave or next pandemic. Future Med Chem. 2021 Oct;13(20):1713-1715. doi: 10.4155/fmc-2021-0209. Epub 2021 Sep 2. PMID: 34472366; PMCID: PMC8438928.
17. Sharma MD, Pacholczyk R, Shi H, Berrong ZJ, Zakharia Y, Greco A, Chang CS, Eathiraj S, Kennedy E, Cash T, Bollag RJ, Kolhe R, Sadek R, McGaha TL, Rodriguez P, Mandula J, Blazar BR, Johnson TS, Munn DH. Inhibition of the BTK-IDO-mTOR axis promotes differentiation of monocyte-lineage dendritic cells and enhances anti-tumor T cell immunity. Immunity. 2021 Oct 12;54(10):2354-2371.e8. doi: 10.1016/j.immuni.2021.09.005. Epub 2021 Oct 5. PMID: 34614413; PMCID: PMC8516719.
18. Vyavahare S, Kumar S, Cantu N, Kolhe R, Bollag WB, McGee-Lawrence ME, Hill WD, Hamrick MW, Isales CM, Fulzele S. Tryptophan-Kynurenine Pathway in COVID-19-Dependent Musculoskeletal Pathology: A Minireview. Mediators Inflamm. 2021 Oct 5;2021:2911578. doi: 10.1155/2021/2911578. PMID: 34621138; PMCID: PMC8492288.
19. *Sahajpal NS, Mondal AK, Ananth S, Njau A, Fulzele S, Ahaluwalia P, Chaubey A, Hegde M, Rojiani AM, Kolhe R. Making a Difference: Adaptation of the Clinical Laboratory in Response to the Rapidly Evolving COVID-19 Pandemic. Acad Pathol. 2021 Jun 9;8:23742895211023948. doi: 10.1177/23742895211023948. PMID: 34263025; PMCID: PMC8246561.
20. *Ahluwalia P, Vaibhav K, Ahluwalia M, Mondal AK, Sahajpal N, Rojiani AM, Kolhe R. Infection and Immune Memory: Variables in Robust Protection by Vaccines Against SARS-CoV-2. Front Immunol. 2021 May 11;12:660019. doi: 10.3389/fimmu.2021.660019. PMID: 34046033; PMCID: PMC8144450.
21. *Sahajpal NS, Mondal AK, Ananth S, Njau A, Ahluwalia P, Kota V, Caspary K, Ross TM, Farrell M, Shannon MP, Fulzele S, Chaubey A, Hegde M, Rojiani AM, Kolhe R. Clinical Validation of a Sensitive Test for Saliva Collected in Healthcare and Community Settings with Pooling Utility for Severe Acute Respiratory Syndrome Coronavirus 2 Mass Surveillance. J Mol Diagn. 2021 Jul;23(7):788-795. doi: 10.1016/j.jmoldx.2021.04.005. Epub 2021 May 4. PMID: 33957320; PMCID: PMC8129802.
22. *Sahajpal NS, Mondal AK, Ananth S, Njau A, Ahluwalia P, Newnam G, Lozoya-Colinas A, Hud NV, Kota V, Ross TM, Reid MD, Fulzele S, Chaubey A, Hegde M, Rojiani AM, Kolhe R. SalivaSTAT: Direct-PCR and Pooling of Saliva Samples Collected in Healthcare and Community Setting for SARS-CoV-2 Mass Surveillance. Diagnostics (Basel). 2021 May 19;11(5):904. doi: 10.3390/diagnostics11050904. PMID: 34069462; PMCID: PMC8159081.
23. *Ahluwalia P, Ahluwalia M, Mondal AK, Sahajpal NS, Kota V, Rojiani MV, Kolhe R. Natural Killer Cells and Dendritic Cells: Expanding Clinical Relevance in the Non-Small Cell Lung Cancer (NSCLC) Tumor Microenvironment. Cancers (Basel). 2021 Aug 11;13(16):4037. doi: 10.3390/cancers13164037. PMID: 34439191; PMCID: PMC8394984.
24. Boyle TA, Mondal AK, Saeed-Vafa D, Ananth S, Ahluwalia P, Kothapalli R, Chaubey A, Roberts E, Qin D, Magliocco AM, Rojiani AM, Kolhe R. Guideline-Adherent Clinical Validation of a Comprehensive 170-Gene DNA/RNA Panel for Determination of Small Variants, Copy Number Variations, Splice Variants, and Fusions on a Next-Generation Sequencing Platform in the CLIA Setting. Front Genet. 2021 May 20;12:503830. doi: 10.3389/fgene.2021.503830. PMID: 34093633; PMCID: PMC8172991.
25. *Sahajpal NS, Barseghyan H, Kolhe R, Hastie A, Chaubey A. Optical Genome Mapping as a Next-Generation Cytogenomic Tool for Detection of Structural and Copy Number Variations for Prenatal Genomic Analyses. Genes (Basel). 2021 Mar 11;12(3):398. doi: 10.3390/genes12030398. PMID: 33799648; PMCID: PMC8001299.
26. *Ahluwalia P, Ahluwalia M, Mondal AK, Sahajpal N, Kota V, Rojiani MV, Rojiani AM, Kolhe R. Prognostic and therapeutic implications of extracellular matrix associated gene signature in renal clear cell carcinoma. Sci Rep. 2021 Apr 7;11(1):7561. doi: 10.1038/s41598-021-86888-7. PMID: 33828127; PMCID: PMC8026590.
27. Kolhe R, Sharma A, Lee TJ, Zhi W, Ghilzai U, Mondal AK, Liu Y, Isales CM, Hamrick MW, Hunter M, Fulzele S. Sex-Specific Differences in Extracellular Vesicle Protein Cargo in Synovial Fluid of Patients with Osteoarthritis. Life (Basel). 2020 Dec 10;10(12):337. doi: 10.3390/life10120337. PMID: 33321751; PMCID: PMC7763294.
28. *Ahluwalia P, Ahluwalia M, Mondal AK, Sahajpal N, Kota V, Rojiani MV, Rojiani AM, Kolhe R. Immunogenomic Gene Signature of Cell-Death Associated Genes with Prognostic Implications in Lung Cancer. Cancers (Basel). 2021 Jan 5;13(1):155. doi: 10.3390/cancers13010155. PMID: 33466402; PMCID: PMC7795632.
29. *Ahluwalia P, Kolhe R, Gahlay GK. The clinical relevance of gene expression-based prognostic signatures in colorectal cancer. Biochim Biophys Acta Rev Cancer. 2021 Apr;1875(2):188513. doi: 10.1016/j.bbcan.2021.188513. Epub 2021 Jan 22. PMID: 33493614.
30. Zakharia, Yousef, Robert R. McWilliams, Olivier Rixe, Joseph Drabick, Montaser F. Shaheen, Kenneth F. Grossmann, Ravindra Kolhe et al. “Phase II trial of the IDO pathway inhibitor indoximod plus pembrolizumab for the treatment of patients with advanced melanoma.” Journal for ImmunoTherapy of Cancer 9, no. 6 (2021): e002057.
31. Padala SA, Barsouk A, Barsouk A, Rawla P, Vakiti A, Kolhe R, Kota V, Ajebo GH. Epidemiology, Staging, and Management of Multiple Myeloma. Med Sci (Basel). 2021 Jan 20;9(1):3. doi: 10.3390/medsci9010003. PMID: 33498356; PMCID: PMC7838784.
32. Tran, P.M.H., Tran, L.K.H., Satter, K.B., Purohit, S., Nechtman, J., Hopkins, D.I., Dos Santos, B., Bollag, R., Kolhe, R., Sharma, S. and She, J.X., 2021. Retrospective Validation of a 168-Gene Expression Signature for Glioma Classification on a Single Molecule Counting Platform. Cancers, 13(3), p.439.
33. Singh, G., Bollag, R., Kolhe, R., McMullen, A., & Savage, N. M. (2021). Engaging Pathology Residents in Scholarly Activities. The Journal of Applied Laboratory Medicine, 6(2), 567-569.
34. Fulzele S, Sahay B, Yusufu I, Lee TJ, Sharma A, Kolhe R, Isales CM. COVID-19 Virulence in Aged Patients Might Be Impacted by the Host Cellular MicroRNAs Abundance/Profile. Aging and disease. 2020 Jun;11(3):509.
35. Jarrahi, Abbas, Meenakshi Ahluwalia, Hesam Khodadadi, Evila da Silva Lopes Salles, Ravindra Kolhe, David C. Hess, Fernando Vale et al. “Neurological consequences of COVID-19: what have we learned and where do we go from here?.” Journal of neuroinflammation 17, no. 1 (2020): 1-12.
36. Tran, P.M., Tran, L.K., Nechtman, J., Dos Santos, B., Purohit, S., Satter, K.B., Dun, B., Kolhe, R., Sharma, S., Bollag, R. and She, J.X., 2020. Comparative analysis of transcriptomic profile, histology, and IDH mutation for classification of gliomas. Scientific Reports, 10(1), pp.1-13.
37. *Ahluwalia P, Ahluwalia M, Vaibhav K, Mondal A, Sahajpal N, Islam S, …& , Kolhe R. Infections of the lung: a predictive, preventive and personalized perspective through the lens of evolution, the emergence of SARS-CoV-2 and its pathogenesis. EPMA J. 2020 Nov 13;11(4):1-21. doi: 10.1007/s13167-020-00230-1. Epub ahead of print. PMID: 33204369; PMCID: PMC7661834.
38. *Sahajpal NS, Mondal AK, Njau A, Ananth S, Ghamande S, Hegde M, Chaubey A, M Rojiani A, Kolhe R. COVID-19 screening in a healthcare or community setting: complexity of saliva as a specimen for PCR-based testing. Future Med Chem. 2021 Jan;13(1):9-12. doi: 10.4155/fmc-2020-0255. Epub 2020 Nov 24. PMID: 33228389; PMCID: PMC7684981.
39. *Sahajpal NS, Mondal AK, Ananth S, Njau A, Ahluwalia P, Jones K, Ahluwalia M, Okechukwu N, Savage NM, Kota V, Rojiani AM, Kolhe R. Clinical performance and utility of a comprehensive next-generation sequencing DNA panel for the simultaneous analysis of variants, TMB and MSI for myeloid neoplasms. PLoS One. 2020 Oct 19;15(10):e0240976. doi: 10.1371/journal.pone.0240976. PMID: 33075099; PMCID: PMC7571681.
40. *Sahajpal NS, Mondal AK, Njau A, Ananth S, Jones K, Ahluwalia PK, Ahluwalia M, Jilani Y, Chaubey A, Hegde M, Kota V, Rojiani A, Kolhe R. Proposal of RT-PCR-Based Mass Population Screening for Severe Acute Respiratory Syndrome Coronavirus 2 (Coronavirus Disease 2019). J Mol Diagn. 2020 Oct;22(10):1294-1299. doi: 10.1016/j.jmoldx.2020.07.001. Epub 2020 Jul 30. PMID: 32738298; PMCID: PMC7391280.
41. *Sobash, P. T., Kolhe, R., Karim, N. A., Guddati, A. K., Jillella, A., & Kota, V. (2020). Role of indoleamine 2, 3-dioxygenase in acute myeloid leukemia. Future Oncology, 16(36), 3085-
3094.
42. *S Sahajpal N, Mondal AK, Njau A, Ananth S, Jones K, Ahluwalia PK, Ahluwalia M, Jilani Y, Chaubey A, Hegde M, Kota V, Rojiani A, Kolhe R. Effective optimization of SARS-CoV-2 laboratory testing variables in an era of supply chain constraints. Future Microbiol. 2020 Nov 12:1483-1487. doi: 10.2217/fmb-2020-0094. Epub ahead of print. PMID: 33179525.
43. *Sahajpal NS, Njau A, Mondal AK, Ananth S, Chaubey A, Rojiani A, Kolhe R. Role of clinical laboratories in response to the COVID-19 pandemic. Future Med Chem. 2020 Sep;12(17):1521-1523. doi: 10.4155/fmc-2020-0129. Epub 2020 Jun 19. PMID: 32552003; PMCID: PMC7342020.
44. *Cain, M., Awad, M. E., Kolhe, R., Mondal, A. K., Ghilzai, U., Isales, C., ... & Fulzele, S. (2020).
Dysregulation of epigenetic related genes in Diabetic Trigger finger Patients; preliminary analysis of Patient-Derived Samples. Biomolecular Concepts, 11(1), 221-229.
45. *Singleton, Q., Vaibhav, K., Braun, M., Patel, C., Khayrullin, A., Mendhe, B., Lee, B.R., Kolhe, R., Kaiser, H., Awad, M.E. and Fariyike, T., 2019. Bone marrow derived extracellular vesicles activate osteoclast differentiation in traumatic brain injury induced bone loss. Cells, 8(1), p.63.
46. *Kaufman ME, Miller DT, Ullah A, White J, Singh G, Kolhe R, Williams H, Mittal P, Parikh J, Terris MK. Skene’s Gland Adenocarcinoma: Borrowing From Prostate Cancer Experience for the Evaluation and Management of a Rare Malignancy. Urology. 2020 Jun 3.
47. *Xiao, W., Wang, L., Howard, J., Kolhe, R., Rojiani, A. M., & Rojiani, M. V. (2019). TIMP-1-Ajebo Mediated Chemoresistance via Induction of IL-6 in NSCLC. Cancers, 11(8), 1184.
48. *Dalton, S., Smith, K., Singh, K., Kaiser, H., Kolhe, R., Mondal, A. K., ... & Fulzele, S. (2019). Accumulation of kynurenine elevates oxidative stress and alters microRNA profile in human bone marrow stromal cells. Experimental Gerontology, 110800.
49. Gupta, V. R., Giller, C., Kolhe, R., Forseen, S. E., & Sharma, S. (2019). Polymorphous Low-Grade Neuroepithelial Tumor of the Young: A Case Report with Genomic Findings. World neurosurgery, 132, 347-355.
50. Munjal, A., Bapat, S., Hubbard, D., Hunter, M., Kolhe, R., & Fulzele, S. (2019). Advances in Molecular biomarker for early diagnosis of Osteoarthritis. Biomolecular concepts, 10(1), 111-119.
51. Periyasamy-Thandavan, S., Burke, J., Mendhe, B., Kondrikova, G., Kolhe, R., Hunter, M., ... & Fulzele, S. (2018). MicroRNA-141-3p Negatively Modulates SDF-1 Expression in Age-Dependent Pathophysiology of Human and Murine Bone Marrow Stromal Cells. The Journals of Gerontology: Series A.
52. Ahluwalia, P., Mondal, A.K., Bloomer, C., Fulzele, S., Jones, K., Ananth, S., Gahlay, G.K., Heneidi, S., Rojiani, A.M., Kota, V. and Kolhe, R., 2019. Identification and Clinical Validation of a Novel 4 Gene-Signature with Prognostic Utility in Colorectal Cancer. International journal of molecular sciences, 20(15), p.3818.
53. Alwhaibi, A., Kolhe, R., Gao, F., Cobran, E. K., & Somanath, P. R. (2019). Genome atlas analysis based profiling of Akt pathway genes in the early and advanced human prostate cancer. Oncoscience, 6(5-6), 317.
54. Dimri, Manali, Ashley Humphries, Archana Laknaur, Sawsan Elattar, Tae Jin Lee, Ashok Sharma, Ravindra Kolhe, and Ande Satyanarayana. “NAD (P) H Quinone Dehydrogenase 1 Ablation Inhibits Activation of the Phosphoinositide 3-Kinase/Akt Serine/Threonine Kinase and Mitogen-Activated Protein Kinase/Extracellular Signal-Regulated Kinase Pathways and Blocks Metabolic Adaptation in Hepatocellular Carcinoma.” Hepatology (Baltim.) (2019).
55. Chun, K., Wenger, G.D., Chaubey, A., Dash, D.P., Kanagal-Shamanna, R., Kanturci, S., Kolhe, R., Van Dyke, D.L., Wang, L., Wolff, D.J. and Miron, P.M., 2018. Assessing copy number aberrations and copy-neutral loss-of-heterozygosity across the genome as best practice: An evidence-based review from the Cancer Genomics Consortium (CGC) working group for chronic lymphocytic leukemia. Cancer genetics.